Lifeact-EGFP transgenic mice show similar acetylcholine-induced exocytic responses to wild type.

<p>(<b>A</b>) The images were taken before (0 s) and 300 s after exocytic fusion induced by 1 µM acetylcholine stimulation of mouse pancreatic fragments in wild type (upper images) and Lifeact-EGFP transgenic animals (lower images). The tissue fragments were bathed in extracellular fluorescent dye (SRB) which labels the lumens (bright fluorescence between the cells in control) and enters and labels each individual granule (bright fluorescent spots along the lumen after 300 s). (<b>B</b>) We identify the time point of appearance of each fusing granule (cumulative histogram, aligned to the first exocytic event) which shows both wild type and transgenic animals have a similar time-course and extent of exocytic response. (C) Further, we compare the fluorescence profile, over time, of SRB dye entry into each individual granule and observe no differences between wild type and transgenic animals.