CD25⁺ cell depletion prior to DNA vaccination upregulates both pro- and anti-inflammatory cytokines in pAVX1-Sj26GST-vaccinated mice.

<p>Seven days after the last immunization with pVAX1 or pVAX1-Sj26GST, splenocytes (2×10⁵/well) from each mouse were incubated in triplicate wells for three days in 200 µl of media in 96-well plates in the presence of SWA (15 µg/ml), or control media. Supernatants were collected after 72 h of culture and tested for IFN-γ (A), GM-CSF (B), IL-4 (C), or IL-10 (D). Bars show the mean±SD (n = 6 per group) of 18 mice from three independent experiments performed in triplicate wells. *<i>P</i><0.05; **<i>P</i><0.01.