<i>vha-19(RNAi)</i> adults produce fewer oocytes beyond 24 hours of exposure to <i>vha-19</i> dsRNA.

<p>A) The average number of eggs laid every 24 hours by <i>control(RNAi)</i> or <i>vha-19(RNAi)</i> adults feeding on dsRNA. Error bars represent the standard error of the mean (s.e.m). ***indicates p<0.001. B) The average number of oocytes in the proximal oviduct of either wild type or AZ212 (pie-1::H2B::GFP) <i>C. elegans</i> fed on control or <i>vha-19</i> dsRNA for 24 or 48 hours. C) The proximal oviduct of AZ212 <i>C. elegans</i> fed control or <i>vha-19</i> dsRNA for 24 hours. White arrows indicate normal oocyte nuclei. Endomitotic oocytes in the oviduct are indicated by a square bracket. Note that both oocytes with multiple nuclei (most proximal oocyte in square bracket), and oocytes in which the chromosomes have started to decondense prematurely (indicated by the green background of the two most distal oocytes in the square bracket), are endomitotic. The irregular, bright, particulate dots in the distal gonad (dg) are autofluorescent gut granules. Scale bars indicate 10 µm. D) The percentage of endomitotic oocytes observed in the oviducts of wild type or AZ212 <i>C. elegans</i> fed control or <i>vha-19</i> dsRNA from the L4 stage for 24 or 48 hours.