Expression of HS1 protein in CLL B lymphocytes.

<p>The lysates obtained from normal B lymphocytes and leukemic B cells from CLL patients were analyzed by immunostaining with antibody against HS1. Blots were reprobed with anti-β-actin antibody as loading control. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039902#pone-0039902-g001" target="_blank">Figure 1A</a> is representative of four CLL and four healthy subjects with the respective densitometry of HS1/β-actin ratio. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039902#pone-0039902-g001" target="_blank">Figure 1B</a> shows HS1/β-actin ratio of 71 CLL patients and 26 normal controls. Data has been normalized putting equal to 1 the ratio calculated in Jurkat cell line. Data obtained were evaluated for their statistical significance with the Student’s <i>t</i>-test (* p<0.01 between normal controls and CLL patients, <b>B</b>) or ANOVA (** p<0.01 between normal <i>vs</i> mutated CLL <i>vs</i> unmutated CLL, <b>C</b>; normal <i>vs</i> CD38 neg CLL <i>vs</i> CD38 pos CLL, <b>D</b>; normal <i>vs</i> 13q- and normal karyotype CLL <i>vs</i> 17p- and 11q- CLL, <b>E</b>; normal <i>vs</i> treated CLL <i>vs</i> untreated CLL, <b>F</b>; normal <i>vs</i> still alive patients <i>vs</i> dead patients, <b>G</b>). Medians are represented by solid lines. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039902#pone-0039902-g001" target="_blank">Figure 1H</a> represents the overall survival comparison between patients (n = 16) presenting high levels of HS1 (HS1>0.93, dotted line) and patients (n = 54) presenting low levels of HS1 (HS1<0.93, solid line); the difference between curves is statistically significant (p<0.05, Kaplan Meier). 0.93 is the median of HS1 levels in normal controls.