VHA-19 is required for uptake of vitellogenin via trafficking of the RME-2 receptor.

<p>A) VIT-2::GFP <i>C. elegans</i> [DH1033] were fed control or <i>vha-19</i> dsRNA from the L4 stage for 48 hours and the oocytes examined. VIT-2::GFP did not reach the oocytes in adults fed <i>vha-19</i> dsRNA, instead it accumulated between the oocytes (thin arrows). Fat arrows indicate agglomerates of VIT-2::GFP. B) RME-2::GFP was not trafficked to the plasma membrane in RME-2::GFP; <i>vha-19(RNAi)</i> adults (arrows and dotted circle), whereas RME-2::GFP did reach the plasma membrane in worms fed control dsRNA (thin arrows between oocytes). Worms were fed <i>vha-19</i> or control dsRNA for 48 hours. Arrowheads in bright field panels indicate individual oocytes. Scale bars represent 10 µm.