Expression of mRNA and surface protein for components of the IL-4/IL-13 receptor complex on mouse eosinophils.

<p>(A). Expression of mRNA for the different subunits of the IL-4/IL-13 receptor complex. RNA was extracted from purified eosinophils and the mouse macrophage cell line, RAW 264.7, and cDNA was synthesized. Relative quantitation by RealTime PCR with specific primer pairs for each receptor subunit was performed, using the standard 2^−ΔΔCt method, and the products were resolved on a 3% agarose gel. The amount of expressed mRNA for each subunit from mouse eosinophils (filled bars) relative to the amount in the control RAW cell line ( = 1, open bars) was graphed. (B). Cell surface expression of the IL-4/IL-13 receptor subunits on mouse eosinophils. FACS analysis using specific antibodies to each receptor subunit was performed. Representative FACS histograms from one experiment (of four to five independent experiments) are shown with staining with specific antibody (solid black line) and isotype-matched control antibody (dotted line). Receptor staining on mouse bone marrow-derived macrophages from wildtype and γC-deficient mice, generated as described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039673#pone.0039673-Heller1" target="_blank">[12]</a>, and to RAW 264.7 cells is shown for comparison.