Pre-treatment with IL-4 but not IL-13 enhanced migration of purified mouse eosinophils to eotaxin-1 and eotaxin-2.

<p>Eosinophils were purified as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039673#s2" target="_blank">Methods</a>. (A). Eosinophils were then incubated with various concentrations of IL-4 or IL-13 at 37°C for 30 minutes. The treated cells were washed and then placed (5×10⁵) into the upper chamber insert of the Transwell plate; the lower chamber contained either 0 or 30 nM eotaxin-1. The chemotaxis assay was performed and the number of migrated cells was counted as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039673#pone-0039673-g001" target="_blank">Figure 1</a>. A representative experiment is shown. (B) Average data from three independent chemotaxis experiments (<i>n</i> = 3; *<i>P</i><0.05, compared to migration to eotaxin-1 alone). For the IL-4 graph, number of migrated cells to medium = 11,614± SEM 3,257 and to eotaxin-1 = 102,435± SEM 27,964 and for the IL-13 graph, number of migrated cells to medium = 8,581± SEM 2,163 and to eotaxin-1 = 75,651± SEM 20,436. (C, left panel) Time of IL-4 pre-treatment of eosinophils prior to chemotaxis. Eosinophils were pre-treated with 10 ng/mL IL-4 for the indicated times, subjected to chemotaxis to 30 nM eotaxin-1 and counting as in (A). <i>P</i><0.05. (C, right panel) Eosinophils were pre-treated with 10 ng/mL IL-4 for the indicated time, subjected to chemotaxis to 30 nM eotaxin-2 and counting as in (A). <i>P</i><0.05.