Effect of pre-treatment with IL-4 and IL-13 on CCR3 expression and eotaxin-1 signaling in mouse eosinophils.

<p>(A). Eosinophils were treated with (heavy solid line) or without (light solid line) 100 ng/mL IL-4 (left panel) or IL-13 (right panel) for 30 min and FACS analysis with specific antibodies to CCR3 (solid lines), an isotype control (dotted) or unstained (dashed line) was performed as described (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039673#pone-0039673-g001" target="_blank">Figure 1</a>). (B) The cells were serum-starved for 2 hours prior to stimulation with 20 ng/mL IL-4 (“4”) or IL-13 (“13”) for 15 mins or 30 nM eotaxin-1 (“eot”) for 1 minute or no stimulation (“–”) as indicated. Cell lysates were prepared and analyzed by Western blotting as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039673#pone-0039673-g005" target="_blank">Figure 5</a>.