Migration of bone marrow-derived eosinophils (bmEos) from γC^+/+ and γC^−/− mice to eotaxin-1.

<p>Eosinophils were differentiated from the bone marrow of γC^+/+ (black bars) or γC^−/− (open bars) mice <i>in vitro</i> as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039673#s2" target="_blank">Methods</a>. The cells were either untreated or primed with 10 ng/mL IL-4 for 30 min prior to placement into the upper chamber of the Transwell chemotaxis assay. Eotaxin-1 (30 nM) was placed in the lower chamber. Migrated eosinophils from triplicate wells were counted as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039673#pone-0039673-g001" target="_blank">Figure 1</a>. The average data from two experiments performed in triplicate are shown (* <i>P</i><0.05, compared to medium control). For γC^+/+, mean number of cells migrated to medium = 44,567± SEM 10,947; to eotaxin-1 = 155,817± SEM 34,864 and IL-4 pre-treated cells to eotaxin-1 = 217,043± SEM 24,531. For γC^−/−, mean number of cells migrated to medium = 51,640± SEM 12,840; to eotaxin-1 = 149,862± SEM 13,133 and IL-4 pre-treated cells to eotaxin-1 = 145,768± SEM 19,289.