Mucin layer protects TLR4 on intestinal epithelial cells from trypsin-mediated degradation.

<p>Western blot for intra- and extra-cellular domains of E-cadherin of TLR4 cells without a mucin layer after exposure with serum free without and with trypsin (SFM and SFM+Try respectively) and IEC-18 cell with a mucin layer after exposure to serum free media with or without trypsin (SFM+Mucin and SFM+Mucin +Try) (<b>A</b>) with corresponding density levels measurements (<b>B</b>). Values are mean±SEM (n = 4)/group, **P<0.001 compared to SFM, ††P<0.001 compared to SFM+Mucin, and ‡P<0.05compared to SFM+Mucin+Try.