Gem deletion does not alter islet development

<p>. <b>A</b>) Lox-p sites were introduced on either side of exon 2 of the Gem gene. Deletion of exon 2 was confirmed by PCR and by Southern blotting. <b>B</b>) <i>GEM</i> mRNA is highly expressed in human islets compared to other members of the RGK family and to the house-keeping gene TATA-box binding protein (TBP). <b>C</b>) Islet morphology and islet mass (<b>D</b>) were not altered by Gem deletion in mice. <b>E</b>) Total insulin content of the pancreas was not altered by Gem deletion. Black bars indicate Gem^+/+ mice, and white bars indicate Gem-deficient (−/−) mice. Error bars indicate ±1SEM.