Induction of virus-specific IgG and IgA upon immunization with Delta H5N1.

<p>BALB/c mice were immunized with different doses of Delta H5N1 (▴ H5N1) or wt live virus A/PR8/34 (PR8) H1N1 via the sublingual (SL), nasal (N) or intranasal (IN) route. Four weeks later sera were collected and the levels of H5N1 virus-specific IgG and IgA were determined by Delta H5N1 virus-coated ELISA plates. The values represent the mean + SEM (vertical bars) end point ELISA antibody titers determined on 5 mice per group.