Purification of the BoNT/B complex

a<p>The concentration of total BoNT/B complex was determined by a “sandwich” ELISA method using the purified BoNT/B as a standard. And the purified BoNT/B concentration was determined by BCA assay.</p>b<p>Minimum lethal dose (MLD) was defined as the minimum dose required to kill two mice as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039670#s4" target="_blank">Materials and Methods</a>.</p>c<p>The purification factor was estimated by the mouse toxigenicity test of BoNT/B complex.