Human MMs express FOXM1.

<p>A) Paraffin-embedded sections from human MM tissue specimens were examined for FOXM1 expression using immunohistochemistry; shown are representative results for the indicated tumor types at a 1∶3000 dilution of primary antibody, which accentuates nuclear localization of FOXM1. No signal for FOXM1 was observed in normal human liver. B) Nuclear expression of FOXM1 was scored for the indicated tumor types, using 0 = no positive nuclei, 1 = <5% positive nuclei, 2 = >5% and <50% positive nuclei, and 3 = >50% positive nuclei. Significant levels of nuclear FOXM1 expression were observed in all MM tumor types. There was no significant difference in nuclear FOXM1 expression between mesothelioma tumor types. C) Total RNA was extracted from normal mesothelial tissue (N) or mesothelioma (T) from four patients and examined for FOXM1 mRNA expression relative to HPRT using qRT-PCR. Using the Student’s t-test, relative expression (RQ) for FOXM1 transcript in tumor tissue was increased as compared to normal mesothelium (p = 0.0017).