Genetic transformation of banana cv. <i>Rasthali</i> with full-length <b><i>Petunia</i></b><b> defensin constructs.</b>

<p> (A) <i>Petunia</i> flower which served as the source of defensin genes. (B) T- DNA region of p<i>PhDef1</i>-1301/p<i>PhDef2</i>-1301 binary vector wherein <i>PhDef1</i> and <i>PhDef2</i> coding sequences were cloned downstream of <i>Zea mays</i> polyubiquitin promoter and upstream of nos (nopaline synthase) 3′ UTR in MCS of pCAMBIA-1301 vector. (C) and (D) Somatic embryos derived from p<i>PhDef1</i>-1301 and p<i>PhDef2</i>-1301 transformed cells on embryo induction medium 6 weeks post cocultivation. (E) and (F) Germination of transgenic somatic embryos derived from p<i>PhDef1</i>-1301 and p<i>PhDef2</i>-1301 transformed cells on medium supplemented with BAP 10 weeks post cocultivation. (G) and (H) Multiple shoots derived from p<i>PhDef1</i>-1301 and p<i>PhDef2</i>-1301 transformed cells on shoot induction medium 14 weeks post cocultivation. (I) and (J) Individual shoots derived from p<i>PhDef1</i>-1301 and p<i>PhDef2</i>-1301 transformed cells on root induction medium supplemented with NAA. (K) Hardening of rooted transgenic plants in the green house.