The dose-dependent binding of curcuminoids to NT2/D1 cells.

<p>(A) Binding dose curves with variable serum and curcuminoid concentrations in media containing FCS with saturated SOLID(S)-[•, short dash] and DMSO(D)-solubilized [▪, long dash] curcuminoids, and a 1∶1 mixture of the two (S+D) [▾, solid line]. The data points represent values from three independent experiments normalized to cell number. The following additional statistical parameters were calculated: R²-values: 0.99 (S), 0.98 (D), and 0.99 (S+D); K_Ds: 6.27±1.03 µM (S), 7.23±1.54 µM (D), 10.24±1.36 µM (S+D); and the slope m (see equation in text) indicating degree of non-specific binding: 1.9×10⁻³ (S), 2.9×10⁻³ (D), and 3.9×10⁻³ (S+D). (B) Binding dose curves with variable curcuminoid concentrations (S+D) at three constant total serum concentrations (5%, 50%, and 100%). (C) Binding dose curve with constant 47 µM curcuminoid concentration (S+D) and variable serum concentration.