Expression analyses of the transgenic banana plants.

<p>(A) and (B) RT-PCR of selected p<i>PhDef1</i>-1301 and p<i>PhDef2</i>-1301transgenic lines performed using primers specific to <i>PhDef1</i> and <i>PhDef2</i>. (C) and (D) Northern blot analysis of selected p<i>PhDef1</i>-1301 and p<i>PhDef2</i>-1301 transgenic lines performed using FA-MOPS gel. Total RNA isolated from the transgenic leaves was filter hybridized with DIG labeled DNA probes targeted against <i>PhDef1</i> (C) and <i>PhDef2</i> (D) gene. Note the high-level expression of <i>Petunia</i> defensins in the different transgenic lines. rRNA bands stained using ethidium bromide indicate equal loading of total RNA in different lanes.