The comparison of iNOS expression between IL-17A-neutralized mice and IgG2a-treated control mice.

<p>Mice were intranasally infected with 1×10³ IFUs of Cm. For neutralization of airway IL-17A, mice were intranasally administered with 10 µg of anti-mouse IL-17A mAb in 40 µl of PBS at 2 h post-infection (p.i.), followed by subsequent administration of the same dose of Ab at 48-h intervals. Infected control mice were administered intranasally with anti-murine IL-17A Ab isotype control (IgG2a) in the same schedule as anti-IL-17A delivery. Mice were monitored for body weight changes (A) and killed at day 7 after infection. The lungs were collected and analyzed for in vivo chlamydial growth (B) and gene expression (C and D) as described in <i>Materials and Methods</i>. D, densitometric analysis of iNOS mRNA RT-PCR products from lung in mice at day 7 postinfection. Values were normalized to β-actin and expressed as the mean ± SD with n = 5 for each point. **, p<0.01.