Comparison of DimerX, IFNγ-ICS and an in vivo cytotoxicity assay.

<p>OVA₂₅₇-specific CD8⁺ T cells were generated from OT-I mice and increasing numbers were injected i.v. into B6 mice. After 24 hours, naïve B6.SJL splenocytes coated with OVA₂₅₇ (labeled CFSE^high) mixed with uncoated splenocytes (labeled CFSE^low) were injected i.v. into the OT-I T cell-treated B6 mice. After 4 hours, splenocytes were prepared and the percentage of OVA₂₅₇-specific CD8⁺ T cells was determined by DimerX and/or IFNγ-ICS and OVA₂₅₇–specific killing measured by comparing recovery of CD45.1⁺ CFSE^high and CFSE^low cells. Two experiments are shown (A and C) and graphs are labeled with the method used. (B and D) Linear regression analysis and r² statistics for the experiments shown in panels A and C, respectively.