CARM1 regulates the chromatin association of NuRD with a NF-κb-dependent gene.

<p>(A) and (B) The Carm1(+/+) and Carm1(−/−) MEF cells were treated with or without 10 µg/ml LPS for 4 h. The total RNAs were prepared and analyzed by quantitative RT-PCR for LPS induction of NF-κb-regulated target genes MIP2 and GCSF respectively. (C) and (D) The ChIP analyses were performed for the MIP2 promoter using Carm1(+/+) and Carm1(−/−) MEF cells treated with or without LPS for 4 h. Note that the association of NuRD complex was determined using antibodies against MTA2 and CHD4 antibodies, as the antibodies against MTA1 and CHD3 used in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034692#pone-0034692-g005" target="_blank">Fig. 5</a> did not work well for ChIP with MEF cells. The percentage of DNA immunoprecipitated with each antibody was calculated relative to the ChIP input DNA. Values are means±SD (standard deviation) derived from three independent ChIP experiments (E) A working model illustrating how CARM1 facilitates dissociation of NuRD and TIF1β from chromatin. CARM1 catalyzes methylation on H3R17 and H3R26. These modifications in the context of acetylation discharge NuRD and TIF1β from chromatin, which in turn protects histone acetylation and enhances transcriptional activation.