gp96MD expression is restricted to mouse basophils.

<p><i>A</i>, Murine mast cells express intact gp96 without evidence for gp96MD. Left panel showed flow profile of bone marrow-derived mast cells. Right panel demonstrates gp96 immunoblot. <i>B</i>, Purified human basophils (left panel) only express full-length gp96 (right panel). <i>C</i>, Human KU812 basophil cell line does not cleave gp96 with and without ER stress induced by tunicamycin (Tu) or thapsigargin (Thap), demonstrated by gp96 immunoblot. <i>D</i>, Western blots for gp96 in the whole cell lysate of KU812 cells with and without ectopic expression of mMCP11.