Caspase activation in response to TRAM and TRAIL.

<p>(A) Processing of caspase-8, -9 and -3 in A-375 and A-375-TS in response to TRAM-34 (40 µM) and TRAIL is shown by Western blotting. Two independent experiments revealed comparable results. (B) Apoptosis (% of sub-G1 cells) in A-375 and A-375-TS is shown in response to TRAIL, TRAM-34 and the pancaspase inhibitor Q-VD-OPh (10 µM, 1 h pretreatment). (C) Cell proliferation was determined by WST-1. (B, C) Mean values and SDs are shown of two independent experiments, each one with triplicates. Statistical significance of abrogated apoptosis and recovered cell proliferation by Q-VD-OPh is indicated (*; p<0.005).