Plasminogen binding assays.

<p>Plates were coated as detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038836#s4" target="_blank">Materials and Methods</a>.(A) Plasminogen (Plg) (0.5 to 0.007732unit/well) binds to fixed recombinant <i>Mycoplasma bovis</i> α-enolase (rMbEno) in a concentration-dependent manner. (B) In a parallel assay, Plg (0.5 to 0.007732/units) binds to whole cell proteins in a concentration-dependent manner. (C) In a competition assay, binding of plasminogen is inhibited by increasing concentrations of anti-rMbEno antiserum (in serial dilutions from 1/100 to 1/6400). (D) Negative control: Plasminogen binding is inhibited by an anti<i>-M. bovis</i> monoclonal antibody. Three independent experiments were performed in triplicate. The error bars indicate the standard deviations from three independent experiments.