Expression of chemokines/cytokines in renal cortex and urine of D₂−/− mice. A

<p>. Expression of Ltα, MCP-2, and NFkB1 mRNA was quantified by qRT-PCR; results were corrected for expression of GAPDH mRNA and expressed as fold change in comparison to their expression in D₂+/+ mice. *P<0.03 vs. D₂+/+ mice. <b>B</b>. Protein expression of MCP-1 (17 kDa) and TNFα protein (25 kDa) was semi-quantified by immunoblotting. Inset shows one set of immunoblots. Results were corrected for expression of actin and expressed as percentage of the expression in D₂+/+ mice, *P<0.02 vs. D₂+/+ mice, n = 5/group. <b>C.</b> Protein expression of IL-6 (25 kDa) and IL-10 (20 kDa) protein semi-quantified by immunoblotting. Results were corrected for expression of actin and expressed as percentage of the expression in D₂+/+, <b>*</b> P<0.05 vs. D₂+/+ mice, n = 5/group Urinary excretion of IL-6 and IL-10 was quantified by ELISA. *P<0.02 vs. D₂+/+ mice, n = 5/group.