Effects of administration of nitrazepam and flumazenil on tail suspension test (TST) immobility time.

<p>Nitrazepam (1 mg/kg) causes a significant increase in immobility time in the TST, which was blocked by flumazenil (10 mg/kg) in both the <i>Usp46</i> mutant (MT) and in the <i>Usp46</i> KO mice (KO). In the wild-type mice (WT), no significant effects were observed with either single administration of nitrazepam, or co-administration of nitrazepam and flumazenil. Data are expressed as mean + S.E.M. for 5–6 mice in each group. *<i>P</i><0.05, **<i>P</i><0.01 (two-way ANOVA with Bonferroni’s test).