Images and densitometry showing increased SBDP120 immunolabeling in the frontal cortex of 3

<p>×<b>Tg-AD mice relative to wild type (Non-Tg) cohorts at 12 and 24 month of age.</b> Panels A–D are low magnification images illustrating the overall pattern of labeling in the forebrain. Labeling in the frontal cortical area was progressively enhanced with aging and transgenic status (E–M). Labeled profiles were hard to detect in the cortex in 12-month old non-Tg mice (E, I), in contrast to 3×Tg-AD mice (G, L). SBDP120 labeling was clearly present in the cortex of 24 month-old non-Tg (F, J) mice, but was dramatically increased in 3×Tg-AD (M) mice. The labeled elements were granular but frequently appeared to be arranged around cell borders in 24 month-old non-Tg and 12 month-old 3×Tg-AD cortex (J, L). In 24 month-old transgenic mice the labeling was aggregated around the cell perimeter as well as being present in the somata of pyramidal cortical neurons (M). Panel N is a psudocolor image with a background cutoff illustrating the age and genotype differences in relative density of SBDP120 reactivity in cortical layer V. These differences are expressed quantitatively in the bar graph (O). *p<0.005. Scale bar = 1 mm in A applying to B-D; equal to 200 µm for E–H and 50 µm for I–M.