Lung myeloperoxidase and N-acetylglucosaminidase activities as measures of neutrophil and macrophage contents respectively.

<p>Macrophage content was determined by NAG activity and neutrophil content was determined by MPO activity as described previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038839#pone.0038839-Savani4" target="_blank">[82]</a> and in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038839#s4" target="_blank">Materials and Methods</a>. Data were segregated according to the molecular size of HA found in the lung and plotted as box and whisker plots with outliers shown as additional dots. Both NAG (A) and MPO (B) activities were significantly higher in animals that had LMW HA. Newborn pigs resuscitated with 100% O₂ and treated with NAC had significantly lower NAG (C) and MPO (D) activities than those without NAC treatment, suggesting that antioxidant treatment decreases the inflammatory response to resuscitation with hyperoxia.