<p>(<b>A</b>) Expression of full length Notch1 (250 Kd) and Trans-Membrane and Intra-Cellular domain (TMIC, 120 Kd) in Cre/lenti- versus GFP/Lenti-transduced <i>Notch1<sup>Flox/Flox</sup></i> MEFs. Knock-out Notch1 in Cre/lenti-transduced <i>Notch1<sup>Flox/Flox</sup></i> MEFs was analyzed by Western blotting assay. β-actin was used as loading control. (<b>B</b>) Rate of cell growth of MEFs in the presence 2% or absence of serum was measured by MTT assay. 5,000 cells/well were plated. Deletion of Notch1 promotes cell growth of MEFs. (<b>C</b>) Deletion of Notch1 increases cell motility of MEFs. The migration of 15 randomly selected cell pairs was tracked over time (20 hours) by time-lapse photography and velocity was calculated with softwar...
<p>Confocal immunofluorescence analysis of Notch-1 (A-C) and Hes-1 (D-F) expression in C2C12 cells i...
B) Western blot analysis of ES cell lysates (50 μg protein). Full length Notch1 was detected by anti...
The Notch signaling pathway is an evolutionarily conserved way of communicating between cells that c...
Fibroblasts are an integral component of stroma and important source of growth factors and extracell...
Fibroblasts are an integral component of stroma and important source of growth factors and extracell...
<p>Notch activation slows down cell growth rate of fibroblasts. Proliferation of transduced FF2441 c...
<p>Notch signaling was up-regulated by over-expressing NICD-GFP adenovirus or suppressed with γ-secr...
<p>(A) Western blotting and quantification of Notch2 (with c-myc tag), P65, p-P65, IκBα and p-IκBα w...
The transmembrane protein Notch is at the core of a signaling pathway that determines a dazzling arr...
<p>(A) Effect of CSA on Notch signaling <i>in vitro</i>. 293T cells were transfected with various co...
<p>(A) Summary of MF20 IF of RD cells treated with two different doses of GSI-IX, PD173074, or cyclo...
<p><b>A.</b> Mononuclear cell cultures were prepared from <i>mdx</i> muscles and cultured under prol...
Notch is a protein that is often highly active in tumours. To activate Notch it must be cleaved and ...
<p>Notch expressing cell lines H460 (A) or A7R5 (B–E) were cotranfected with luciferase reporters an...
<p><b>A,</b> Left panel, Inhibition of Rink-1 cell growth by Notch 1–4 siRNA tested by MTT assay. Th...
<p>Confocal immunofluorescence analysis of Notch-1 (A-C) and Hes-1 (D-F) expression in C2C12 cells i...
B) Western blot analysis of ES cell lysates (50 μg protein). Full length Notch1 was detected by anti...
The Notch signaling pathway is an evolutionarily conserved way of communicating between cells that c...
Fibroblasts are an integral component of stroma and important source of growth factors and extracell...
Fibroblasts are an integral component of stroma and important source of growth factors and extracell...
<p>Notch activation slows down cell growth rate of fibroblasts. Proliferation of transduced FF2441 c...
<p>Notch signaling was up-regulated by over-expressing NICD-GFP adenovirus or suppressed with γ-secr...
<p>(A) Western blotting and quantification of Notch2 (with c-myc tag), P65, p-P65, IκBα and p-IκBα w...
The transmembrane protein Notch is at the core of a signaling pathway that determines a dazzling arr...
<p>(A) Effect of CSA on Notch signaling <i>in vitro</i>. 293T cells were transfected with various co...
<p>(A) Summary of MF20 IF of RD cells treated with two different doses of GSI-IX, PD173074, or cyclo...
<p><b>A.</b> Mononuclear cell cultures were prepared from <i>mdx</i> muscles and cultured under prol...
Notch is a protein that is often highly active in tumours. To activate Notch it must be cleaved and ...
<p>Notch expressing cell lines H460 (A) or A7R5 (B–E) were cotranfected with luciferase reporters an...
<p><b>A,</b> Left panel, Inhibition of Rink-1 cell growth by Notch 1–4 siRNA tested by MTT assay. Th...
<p>Confocal immunofluorescence analysis of Notch-1 (A-C) and Hes-1 (D-F) expression in C2C12 cells i...
B) Western blot analysis of ES cell lysates (50 μg protein). Full length Notch1 was detected by anti...
The Notch signaling pathway is an evolutionarily conserved way of communicating between cells that c...