Differentiation of bmMPCs into adipocytes.

<p>bmMPCs were cultured in 5 mmol/L (control glucose) or 25 mmol/L (high glucose; HG) glucose for 7 days prior to differentiation and assessed for induction of PPARγ2 and C/EBPα (A). HG-treated bmMPCs increased adipogenesis at day 7, as demonstrated by upregulated PPARγ2 levels (B) and C/EBPα (C). Analysis of cells at day 14 showed increased PPARγ2 upon differentiation but no differences between control- and HG-treated cells. C/EBPα levels (E), on the other hand, were significantly higher in HG-treated cells at day 14 as compared to control glucose treated cells [*p<0.05 compared to control media; †p<0.05 compared to cells treated with control glucose + differentiation media].