G1 pretreatment on the contraction response to PE in diabetic OVX rats.

<p>(<b>A</b>) There was no difference on contraction of thoracic aorta between OVX and diabetic OVX groups. G1 (3 µmol/L) treatment dilated the thoracic arteries in the presence of PE (1 mM) in diabetic OVX rats. L–NAME (100 µM) and G15 (1 µM) abolished the effect of G1 in the diabetic OVX rats as shown by the maximal response (E_max) to PE. The sensitivity of arteries to PE (pD₂) did not change among groups. (<b>B</b>) The eNOS phosphorylation was significantly reduced in OVX diabetic group as compared with OVX group. G1 treatment increased the eNOS phosphorylation; whereas, G15 blocked the eNOS phosphorylation induced by G1. n = 6–12 rings from 4–6 rats.^*<i>p</i><0.05 compared with OVX rats; ^##<i>p</i><0.01 compared with diabetic OVX+G1 rats; ^$$<i>p</i><0.01 compared with diabetic OVX rats. Results are given as the mean ± SEM of three independent experiments.