Effects of miR-125b on cell migration in MCF-7 and MDA-MB-231 in vitro.

<p>(A) Expression of miR-125b in MCF-7, MDA-MB-435, MCF-7/ADR, MDA-MB-231and MCF-10A cells measured by real-time RT-PCR. The normalized miR-125b expression for MCF-10A was set 1. Data were present as mean ± SEM, n = 3, *p<0.05, **p<0.01 vs. MCF-10A. (B) Effects of miR-125b on MCF-7 cell migration in vitro. Movement of MCF-7 and MDA-MB-231 cells into the wound was shown for mimics,inhibitor tranfected and untranfected cells and quantification of the wound healing assay. Data are presented as mean±SEM of three separate experiments, n = 3**p<0.01 vs. control group. (C) Effects of miR-125b on adhesion to fibronectin of MCF-7 and MDA-MB-231 cells. Data were present as mean ± SEM, n = 3, *p<0.05 vs. NC group in MCF-7 and #p<0.05 vs. NC group in MDA-MB-231 cell lines.