Reduced pyrophosphate alters gene expression in cervical cementoblasts.

<p><i>Opn</i> mRNA is markedly increased in root-lining cementoblasts in both (B) <i>Ank</i> and (C) <i>Enpp1</i>^−/−, compared to (A) <i>Ank</i> and <i>Enpp1</i>^+/+ controls. Increased numbers of cells associated with the thick cervical cementum express <i>Dmp1</i> in (E) <i>Ank</i> and (F) <i>Enpp1</i>^−/− molars, compared to (D) ^+/+ controls. <i>Bsp</i> gene expression was not different in cementoblasts in (H) <i>Ank</i> and (I) <i>Enpp1</i>^−/− vs. (G) ^+/+ controls. Black arrowheads indicate regions of positively stained cells. All panels are samples from mice at 14 dpn. Abbreviations: d = dentin, c = (cervical) cementum; p = periodontal ligament; b = bone. Scale bar = 100 µm.