Schematic of the prefrontal cortex (PFC) demonstrating location of tissue punches and neurons used for electrophysiology experiments and morphological analysis.

<p><i>Top:</i> Tissue punches used for western blot analysis centered on the prelimbic (PrL) PFC. Shown are sections of the mouse brain at Bregma 2.00 mm. <i>Bottom</i>: The box shows a bright-field image of a biocytin-labeled layer V pyramidal neuron in the PrL PFC. The inserts show a typical arrangement of the recording and theta-glass stimulation electrode, which was placed within the same layer as the recorded neuron near the basal dendrites.