Dopamine induced cell death in PINK1 ko midbrain cultures.

<p>a: 24 hr incubation of cells with 50 µM dopamine resulted in a significant increase in cell death in PINK1 ko cultures compared to wt cultures (measured by proportion of cells stained with propidium iodide.). b: Co-application of dopamine with mitochondrial substrates malate, pyruvate, or methylsuccinate, or antioxidants DPI, MnTBAP, or selegiline, or verapamil resulted in a reduction in dopamine induced cell death in both PINK1 ko and wildtype cells. For the wt cultures, the reduction in dopamine induced cell death was statistically significant for DPI, MnTBAP, pyruvate and verapamil (compared to wt cultures treated only with dopamine). For the PINK1 ko cultures, the reduction in dopamine induced cell death was statistically significant for DPI, selegiline, MnTBAP, pyruvate, me-succinate, malate, CsA and verapamil (compared to PINK1 ko cultures treated with dopamine alone).