Partial contribution of TLR2 in <i>M. cat</i> induced host response.

<p>BMMØ were generated from TLR2-deficient and WT control mice (C57BL/6J) and cultured in 24 well tissue culture plate at 2×10⁵ cells/well. Cells were then treated with LOS (100 ng/ml), LPS (100 ng/ml), Pam3 (10 µg/ml), HK and live <i>M.cat</i> as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037610#s2" target="_blank">materials and methods</a> for 6 h. Supernatants were collected and the levels of TNF-α (A) and IL-6 (B) were measured by ELISA. Representative data from two independent experiments are shown.