Reduced cytokines production in lung from TLR4 mutant mice in response to live <i>M.cat in vivo</i>.

<p>TLR4 mutant mice C3H/HeJ and wild type control mice C3H/OuJ were challenged with live <i>M.cat</i> type A strain at 1×10⁸ CFU/ml in an aerosol chamber as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037610#s2" target="_blank">materials and methods</a>. Lungs were collected at 3, 6, 12 and 24 h, homogenized and levels of TNF-α (A) and IL-6 (B) were measured by ELISA. (n = 6 for each time point).