Effect of Osthole Treatment on the Tumorigenicity of HCC Cells.

<p>A total of 2×10⁶ SMMC-7721 or Hepa1-6 cells were inoculated subcutaneously (s.c.) into the right flank of nude mice or C57/BL6 mice. Mice were randomized into five groups including osthole treatment (244 mg/kg, 122 mg/kg and 61 mg/kg), corn oil alone as the blank control and cisplatin (5 mg/kg) as the positive control on Day8 and were treated once every other day for 2 weeks. (A) The tumor volumes of the nude mice inoculated with SMMC-7721 cells were measured and calculated once every three days. The tumor sizes on day 21 were shown in the inserted figure. (B) Tumor weights of the nude mice inoculated with SMMC-7721 cells were measured on day21. (C) The tumor volumes of the C57/BL6 mice inoculated with Hepa1-6 cells were measured and calculated once every three days. (D) Tumor weights of the C57/BL6 mice inoculated with Hepa1-6 cells were measured on day21. (E) The body weights of nude mice inoculated with SMMC-7721 cells were weighed on day 21. The (F) spleen weights and (G) thymus weights of the C57/BL6 mice inoculated with Hepa1-6 cells were weighed on day21. Each data point represented the mean±S.D. of 10 mice. Data shown were the representatives of three experiments.