Determination of Sare0718 substrate specificity among the 20 proteinogenic amino acids.

<p>(A) Schematic representation of the substrate screening by malachite green-ammonium molybdate colorimetric assay. Control: the reaction system without Sare0718 (compared with the below Ala sample well); Well 1–19: reaction systems with the substrate of Gly, Val, Leu, Ile, Ser, Thr, Cys, Met, Asp, Glu, Arg, Lys, His, Pro, Phe, Trp, Asn, Gln and Tyr, respectively. (B) Relative activities (substrate specificity) of recombinant Sare0718 for 20 proteinogenic amino acids. Individual substrate activities are presented by bars and the highest activity defined as 100%.