rhcmvIL-10 antibodies neutralized rhcmvIL-10 activity in stimulated PBMC.

<p>rhcmvIL-10 neutralization was determined by incubating LPS-stimulated PBMC with either rhcmvIL-10 and RhCMV seropositive plasma, or rhcmvIL-10 only. The % difference of PBMC IL-12 secretions in the rhcmvIL-10/ plasma versus plasma only (measured by ELISA) was termed “% IL-12 induction restored”. (A) Seropositive plasma sample neutralization of rhcmvIL-10 resulted in a range of 0–100% IL-12 induction restored with a significant difference between the RhCMV seropositive and seronegative samples (p = 0.0083; two-tailed student's t-test). The relative relation between %IL-12 restored and strength of NAB is shown on the right. (B) % IL-12 induction restored was found to be directly correlated to rhcmvIL-10 antibody titers (measured in RU) in rhesus macaque seropositive plasma (Pearson r = 0.8292. p<0.0001).