Lipolytic activity.

<p>A: Ex-vivo lipolysis assay in murine gonadal adipose tissue explants as a marker of fat-tissue specific lipolytic activity, measured as release of FFA after stimulation with forskolin. Shown is the release of FFA in female/male mice before/after weight reduction [n = 7 mice/group, two-way ANOVA] <b>B</b>: Serum concentration of free glycerol in female/male mice before/during weight loss (day 3 of CR) [n = 10 mice/group, two-way ANOVA] ] (factor interaction: p_{sex/weight loss}<0.05). <b>C</b>: Mean RER during day time (6 a.m.–6 p.m.) in females/males measured during weight loss [n = 10 mice/group, unpaired <i>t</i>-test]. <b>D</b>: Analysis of ATGL, HSL, and LPL mRNA expression in gonadal-AT from female/male mice after weight reduction. Data are presented as <i>x</i>-fold expression of females [n = 8−10 mice/group, unpaired <i>t</i>-test, genes were analyzed separately]. Black columns and symbols represent male mice; white: females. DIO: before weight reduction; restriction: during restrictive feeding phase; -20%: after weight reduction, at target weight. *p≤0.05; **p≤0.01 DIO vs. −20% or vs. other sex.