Decreased resistance to a heat shock in cells expressing polyQ-expanded Htt.

<p><b>A</b>) Caspase assay of cells that were grown at 33°C and of heat shocked cells (six hours at 42°C). In either medium containing high concentrations of glucose and serum (HH) or lower concentration of glucose and serum (LL). The error bars represent SDs. Results from four independent experiments were analyzed. * p<0.1 (two tailed t-test). <b>B</b>) Relative caspase activities (from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037929#pone-0037929-g001" target="_blank">Figure 1</a> A). The values for non heat shocked cells were set as 1 and the other signals were calculated accordingly. <b>C</b>) Viability assay (luciferase activity, Promega) of STHdh(Q7) and STHdh(Q111) cells that were grown at 33°C and of heat shocked cells (six hours at 42°C). The signals from STHdh(Q7) cells grown at 33°C was set as 100% and the other signals were calculated accordingly. The error bars represent SDs. Results from four independent experiments were analyzed. * p<0.05 (two tailed t-test). <b>D</b> MTT assay of STHdh(Q7) and STHdh(Q111) cells that were grown at 33°C and of heat shocked cells (six hours at 42°C). The signals from STHdh(Q7) cells grown at 33°C was set as 100% and the other signals were calculated accordingly. The error bars represent SDs. Results from four independent experiments were analyzed. * p<0.05 (two tailed t-test). Experiments shown under B), C), and D) were carried out using LL medium.