Decreased resistance to inhibition of the proteasome or Hsp90 in cells expressing polyQ-expanded Htt.

<p><b>A</b>) Immunofluorescence microscopy of STHdh(Q7) and STHdh(Q111) cells that were treated for six hours with the Hsp90 inhibitor radicicol (5 µM) or the proteasome inhibitor MG132 (10 µM) using anti-Hsp70 and anti-Hsp27 antibodies. The scale bars represent 75 µm <b>B</b>) Western blots prepared from protein lysates treated as in A. <b>C</b>) Viability assay (luciferase activity, Promega) of STHdh(Q7) and STHdh(Q111) cells that were treated with DMSO (control), radicicol (5 µM), or MG132 (10 µM) for six hours. The error bars represent SD. Results from four independent experiments were analyzed. * p<0.001 and **p<0.005 (two tailed t-test).