Ezrin interacts with the E3 ubiquitin ligase WWP1.

<p>(A) Schematic representation of the structural organization of ezrin and WWP1. (B) Lysates from 293T cells transfected with Flag-WWP1 were incubated with immobilized GST or GST fused to full length, N-ter or C-ter ezrin and the blot was performed with the anti-Flag antibody. Lower panel: the blot was stained with ponceau S to reveal the GST-tagged proteins. (C) An extract of 293T cells transfected with Flag-WWP1 was incubated with immobilized GST or GST fused to ezrin wild type or carrying mutations in the PPVY motif. Lower panel: Ponceau S staining of the blot. (D) Extract of cells transfected with Flag-WWP1 was incubated with immobilized GST or GST fused to the C-ter domain of ezrin either wild type or carrying the mutation in the PPVY motif. Lower panel: Coomassie staining of GST tagged proteins.