Ventral side of excised brain from adult transgenic medaka expressing GnRH3:GFP.

<p>(A) Anterior view of the brain showing the location of the olfactory bulbs (OB), bilateral clusters of GnRH:GFP neurons in the terminal nerve (TN), and telencephalon (TELE). (B) Higher magnification of the TN-GnRH3:GFP cluster in the box shown in panel A. In this example, the recording electrode was filled with fluorescent dye (Alexa Fluor 594, A-10442, Invitrogen, Carlsbad CA, USA) in order to better visualize the tip of the electrode relative to the neurons. All electrophysiological recordings were performed on GnRH3:GFP neurons in the TN clusters.