F_max in skinned fibers from muscle stimulated <i>in vivo</i>.

<p><i>A</i>: Mean F_max (+s.e.m.) in skinned fibers from muscles stimulated <i>in vivo</i> (STIM) or from non-stimulated contralateral muscles (CC). F_max in STIM fibers significantly lower than in CC (fibers from 5 rats, <i>P</i><0.05). <i>B</i>: F_max in skinned fibers from muscles stimulated in vivo or from contralateral controls (CC, open bar). Alternate fibers from the stimulated muscles were examined i) without additional treatment (STIM, filled bar) or ii) after treatment with DTT (10 mM, 10 min) prior to being activated (STIM+DTT, 5, dashed bar). No statistically significant differences were found (<i>P</i>>0.05). <i>C</i>: Hill curves fit to averaged force data at each pCa for all fibers for given treatment. Filled squares and solid line: fibers from stimulated muscles (n = 12); open circles and dashed line: fibers from resting contralateral muscles (n = 13). Error bars depict s.e.m. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035226#pone-0035226-t002" target="_blank">Table 2</a> for details of characteristics of Hill curves fitted to fibers individually. No differences were evident.