Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.

<p>PBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTR_inh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTR_inh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTR_inh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).