CDK6 mRNA, protein levels and pRb phosphorylation in lymphoblasts from control and c.709-1G>A carriers individuals.

<p>Immortalized lymphocytes from control and c.709-1G>A carriers, FTLD patients or asymptomatic individuals were seeded at an initial density of 1×10⁶/ml and incubated in serum-free RPMI medium. 48 hours later cells were harvested to isolate RNA and to prepare cell lysates. A: CDK6 mRNA expression levels were analyzed by quantitative RT-PCR (left panel), and the CDK6 protein content was analyzed by WB (right panel). The inmunoblot shows two different cell extracts from control, asymptomatic and FTLD patients is shown. The data represent the mean±SE for six observations in different cell lines. *p<0.05 significantly different from control cells. B: Representative inmunoblots showing pRb and p130 phosphorylation status in two different control, asymptomatic or FTLD individuals is shown. pp = the hyperphosphorylated form of the pRb or the p130 protein. Below it is shown the densitometric analysis of the hyperphosphorylated form of pRb and p130. Data represent the mean±SE for six independent observations in different cell lines. C: Representative immunoblots showing the cellular content of Cyclin D1, D2 and D3, and the CDK inhibitors p16 y p18 in two different control, asymptomatic or FTLD individuals.