Effects of local knockdown of 14-3-3ζ in the CeA on alcohol consumption. <i>A</i>

<p>Local knockdown of 14-3-3ζ in the CeA using the 1854 shRNA increased intake of a 10% alcohol solution (v/v). <b><i>B</i></b> Infection with the less effective 1222 shRNA also increased alcohol intake of a 10% alcohol solution (v/v), but less prominently so than the 1854 14-3-3ζ shRNA. <b><i>C</i></b> In a separate batch of mice, local knockdown of 14-3-3ζ in the CeA using the 1854 shRNA increased alcohol intake of a 15% alcohol solution (v/v) and <b><i>D</i></b> local knockdown of 14-3-3ζ in the CeA using the 1854 shRNA caused persistent preference for the alcohol solution despite adulteration with the bitter tastant quinine. In <b><i>E</i></b> the sites of viral infection in the brain are summarized. The black ellipses show the core of the infection site that was consistently targeted across all animals. The areas marked in grey represent less frequent infected sites that include the anterior amygdala, the basolateral amygdala and part of the caudate putamen, along the injection tract. • Control mice; <b>○</b> 14-3-3ζ-specific shRNA treated mice. * P<0.05 from controls; # P<0.05, ## P<0.01 from 0 μM quinine for mice treated with control lentivirus; $ P <0.05 from 0 μM quinine for mice treated with the 1854 14-3-3ζ shRNA expressing lentivirus by <i>t</i>-test.