Impaired proliferation and granulation tissue formation in CPST wounds.

<p>Ki67 staining (red) on CPST and WT wound sections at day 7 (A) showed reduced numbers of proliferating cells in the epidermal wound edges and the granulation tissue dermal cells (B). CD45 staining (red) and counts (C and D) on day 3 wound sections showed reduced numbers of inflammatory cells in CPST wound beds (D). α-Smooth-Muscle-Actin staining (αSMA) (red) and counts (E and F) showed reduced numbers of myofibroblasts in CPST wounds. mRNA levels of αSMA in day 3 CPST and WT wounds. Representative photos of wound edges are depicted in A; epidermis is delineated in dashed lines. Representative photos of wound granulation tissue are depicted in (C) and (E). Nuclei were stained with DAPI (blue). n = 7 per group. Scale bars: (A) 100 µm, (C and E) 50 µm. Mann-Whitney test, *<i>P</i><0.05, **<i>P</i><0.01. (B, D, F and G) mean +/−SEM.