VWF∶Ag expression levels following hydrodynamic gene delivery.

<p>VWF-deficient mice were injected with 100 µg of pLIVE-m<i>Vwf</i>, WT or the various O-glycosylation mutants. Plasma was collected 96 hours later and VWF∶Ag levels were measured by ELISA. Data are represented as mean plus or minus SEM. Normal pooled plasma from 20 C57BL/6 mice was used as a reference and set at 100%. Results are expressed as a percentage of a normal murine level. n = 22 for WT cDNA and 4–5 for the various mutants. * p<0.05 calculated with unpaired t-test when comparing the mutant with WT cDNA.